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LearnNB110-96875
antibody from Novus Biologicals
Targeting: MAPK14
CSBP1, CSBP2, CSPB1, Mxi2, p38, PRKM14, PRKM15
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB110-96875 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-96875, RRID:AB_1260785
- Product name
- Mouse Monoclonal p38 alpha Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects approx 38kDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Toll-like receptor 3-mediated inflammation by p38 is enhanced by endothelial nitric oxide synthase knockdown.
Koch SR, Choi H, Mace EH, Stark RJ
Cell communication and signaling : CCS 2019 Apr 15;17(1):33
Cell communication and signaling : CCS 2019 Apr 15;17(1):33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: p38 Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - p38 Antibody (9F12) [NB110-96875] - p38 MAPKinase visualized on a retinal injury.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: p38 Antibody (9F12) [NB110-96875] - analysis using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x.
