LS-C815640

antibody from LSBio

Targeting: MAF c-MAF

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C815640

Provider

LSBio

Product name

c-Maf Antibody (Preservative Free) LS-C815640

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-MAF rabbit polyclonal antibody at 1:500 dilution. Lane A: PC12 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 47 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

MAF was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. 1 uL anti-MAF rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-MAF rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa.