Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C186541 - Provider product page
- Provider
- LSBio
- Product name
- MAPKAPK2 / MAPKAP Kinase 2 Antibody (aa44-55) LS-C186541
- Antibody type
- Polyclonal
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human, Mouse, Simian
- Host
- Goat
- Storage
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- MAPKAPK2 antibody EB11929 and (1.5 ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labeled with rabbit anti-MK2.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- MAPKAPK2 antibody HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with EB11929 (0.5 ug/ml) in the left panel and with (0.5 ug/ml) in the right panel.