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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- AP32810PU-N - Provider product page
- Provider
- OriGene
- Product name
- MAPKAP Kinase 2 (MAPKAPK2) goat polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- MAPKAP Kinase 2 (MAPKAPK2) goat polyclonal antibody, Aff - Purified
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- MAPKAPK2
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ??(0.5 ug/ml) staining of MEF lysates (35ug protein in RIPA buffer), from double KO mice in second and third lanes and rescued by introduction of MK2 gene in second lane. Primary incubation was 2 hour. Detected by chemiluminescence.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with ?(0.5 ug/ml) in the left panel and with -N?(0.5 ug/ml) in the right panel.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ?and -N (1.5 ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp.) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.
- Validation comment
- IP
