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- Antibody Data
- Antigen structure
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- Validations
- Other assay [2]
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- Product number
- STJ72943 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-MAPKAPK2 antibody (44-55) (STJ72943)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-MAPKAPK2 (44-55) is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
PQFHVKSGLQIK- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
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Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- HEK293 overexpressing Mouse MK2 fused to GFP or overexpressing MK5 fused to GFP and probed with STJ72942 (0. 5ug/ml) in the left panel and with STJ72943 (0. 5ug/ml) in the right panel.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ72942 and STJ72943 (1. 5ug) immunoprecipitations from lysates of MK2/MK3 double knockout MEFs, with (third and fifth lanes) and without (fourth and sixth lanes) rescued MK2 expression through retroviral transduction. The corresponding lysates (first and second lane resp. ) were analyzed in parallel in this Western blot labelled with rabbit anti-MK2.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
