Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- 13949-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MAPKAPK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLSNSQGQS PPVPFPAPAP PPQPPTPALP HPPAQPPPPP PQQFPQFHVK SGLQIKKNAI IDDYKVTSQV LGLGINGKVL QIFNKRTQEK FALKMLQDCP KARREVELHW RASQCPHIVR IVDVYENLYA GRKCLLIVME CLDGGELFSR IQDRGDQAFT EREASEIMKS IGEAIQYLHS INIAHRDVKP ENLLYTSKRP NAILKLTDFG FAKETTSHNS LTTPCYTPYY VAPEVLGPEK YDKSCDMWSL GVIMYILLCG YPPFYSNHGL AISPGMKTRI RMGQYEFPNP EWSEVSEEVK MLIRNLLKTE PTQRMTITEF MNHPWIMQST KVPQTPLHTS RVLKEDKERW EDVKEEMTSA LATMRVDYEQ IKIKKIEDAS NPLLLKRRKK ARALEAAALA H (1-400 aa encoded by BC036060)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse colon tissue were subjected to SDS PAGE followed by western blot with 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 13949-1-AP) (MAPKAPK2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); F-actinisstainedusingCL555-phalloidin(red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 13949-1-AP (MAPKAPK2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13949-1-AP (MAPKAPK2 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 13949-1-AP ( MAPKAPK2 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 13949-1-AP ( MAPKAPK2 Antibody) at dilution of 1:50 (under 40x lens).