Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS60300 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- IL 5 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human IL-5.
- Description
- Interleukin 5 (IL-5) is a soluble T cell-derived factor, also known as T cellreplacing factor (TRF), that induces B cell and eosinophil growth and differentiation. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of two chains: an Ï« chain that binds IL-5 with low affinity and a ÉÇ chain that does not bind IL-5, but together with the IL-5 Ï« chain, constitutes the high affinity IL-5 receptor. The cytoplasmic domain of both the Ï« and ÉÇ chains is essential for signal transduction. Specifically, the membrane-proximal proline-rich sequence of the cytoplasmic domain of the IL-5R receptor Ï« subunit, IL-5RÏ«, is critical for the IL-5 induced proliferative response, expression of nuclear proto-oncogenes and tyrosine phosphorylation of cellular proteins, such as JAK1 and JAK2.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of IL-5 pAb at 1:500 dilutionLane1:MCF-7 cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate