Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- MAB605 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Impaired activation of lesional CD8+ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis.
Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Mukherjee S, Sengupta R, Mukhopadhyay D, Braun C, Mitra S, Roy S, Kanti Das N, Chatterjee U, von Stebut E, Chatterjee M
Scientific reports 2019 Jan 24;9(1):762
Scientific reports 2019 Jan 24;9(1):762
Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.
Liao K, Meyer E, Lee TN, Loercher A, Sikkema D
Journal of immunological methods 2017 Feb;441:15-23
Journal of immunological methods 2017 Feb;441:15-23
Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology 2004 Jun;112(2):274-89
Immunology 2004 Jun;112(2):274-89
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of IL-5 in PMA and Ca2+ ionomycin-treated Human PBMCs by Flow Cytometry. Human PBMCs, untreated (light orange filled histogram) or activated with 50 ng/mL PMA and 500 ng/mL Ca2+ ionomycin for 5 hours (dark orange filled histogram), were stained with Human IL-5 Monoclonal Antibody (Catalog # MAB605) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.