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- Antibody Data
- Antigen structure
- References [11]
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- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB405-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse IL-5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- TRFK5
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Response patterns of cytokines/chemokines in two murine strains after irradiation.
Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5.
Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism.
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
Arthropod-derived histamine-binding protein prevents murine allergic asthma.
Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Eosinophils promote resolution of acute peritonitis by producing proresolving mediators in mice.
Yamada T, Tani Y, Nakanishi H, Taguchi R, Arita M, Arai H
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Feb;25(2):561-8
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2011 Feb;25(2):561-8
Evidence that 13-14 di-hydro, 15-keto prostaglandin D(2)-induced airway eosinophilia in guinea-pigs is independent of interleukin-5.
Whelan CJ
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2009 Feb;58(2):103-8
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2009 Feb;58(2):103-8
Ectopic lung transplantation induces the accumulation of eosinophil progenitors in the recipients' lungs through an allergen- and interleukin-5-dependent mechanism.
Xavier-Elsas P, Santos-Maximiano E, Queto T, Mendonça-Sales S, Joseph D, Gaspar-Elsas MI, Vargaftig BB
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2007 Jan;37(1):29-38
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2007 Jan;37(1):29-38
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Wen H, Hogaboam CM, Gauldie J, Kunkel SL
The American journal of pathology 2006 Jun;168(6):1940-50
The American journal of pathology 2006 Jun;168(6):1940-50
Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
Clinical immunology (Orlando, Fla.) 2006 Aug;120(2):220-31
The IgE adjuvant effect of particles: characterisation of the primary cellular response in the draining lymph node.
Nygaard UC, Ormstad H, Aase A, Løvik M
Toxicology 2005 Jan 15;206(2):181-93
Toxicology 2005 Jan 15;206(2):181-93
Arthropod-derived histamine-binding protein prevents murine allergic asthma.
Couillin I, Maillet I, Vargaftig BB, Jacobs M, Paesen GC, Nuttall PA, Lefort J, Moser R, Weston-Davies W, Ryffel B
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3281-6
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3281-6
Marked airway eosinophilia prevents development of airway hyper-responsiveness during an allergic response in IL-5 transgenic mice.
Kobayashi T, Iijima K, Kita H
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5756-63
Journal of immunology (Baltimore, Md. : 1950) 2003 Jun 1;170(11):5756-63
Comparison of effects of anti-IL-3, IL-5 and GM-CSF treatments on eosinophilopoiesis and airway eosinophilia induced by allergen.
Tomaki M, Zhao LL, Sjöstrand M, Lindén A, Ichinose M, Lötvall J
Pulmonary pharmacology & therapeutics 2002;15(2):161-8
Pulmonary pharmacology & therapeutics 2002;15(2):161-8
AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity.
Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ
The American journal of pathology 2002 Apr;160(4):1353-60
The American journal of pathology 2002 Apr;160(4):1353-60
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of IL-5 in Human PBMCs by Flow Cytometry. Human peripheral blood monocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium Ionomycin for 6 hours were stained with Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B) and Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB005). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-5 and Neutralization by Human/Mouse IL-5 Antibody. Recombinant Mouse IL-5 (Catalog # 405-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-5 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human/Mouse IL-5 Monoclonal Antibody (Catalog # MAB405). The ND50 is typically 0.004-0.015 µg/mL.
