MAB605

antibody from R&D Systems

Targeting: IL5 EDF, IL-5, TRF

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB605

Provider

R&D Systems

Product name

Human IL-5 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P05113


Isotype

IgG

Antibody clone number

9906

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Impaired activation of lesional CD8⁺ T-cells is associated with enhanced expression of Programmed Death-1 in Indian Post Kala-azar Dermal Leishmaniasis.

Mukherjee S, Sengupta R, Mukhopadhyay D, Braun C, Mitra S, Roy S, Kanti Das N, Chatterjee U, von Stebut E, Chatterjee M
Scientific reports 2019 Jan 24;9(1):762

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Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.

Liao K, Meyer E, Lee TN, Loercher A, Sikkema D
Journal of immunological methods 2017 Feb;441:15-23

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Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.

Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
The Journal of allergy and clinical immunology 2007 Nov;120(5):1118-25

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Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.

Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology 2004 Jun;112(2):274-89

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-5 in Human Peripheral Blood Lymphocytes. IL-5 was detected in immersion fixed human peripheral blood lymphocytes using Mouse Anti-Human IL-5 Monoclonal Antibody (Catalog # MAB605) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm of PBMCs. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-5 in PMA and Ca²⁺ ionomycin-treated Human PBMCs by Flow Cytometry. Human PBMCs, untreated (light orange filled histogram) or activated with 50 ng/mL PMA and 500 ng/mL Ca²⁺ ionomycin for 5 hours (dark orange filled histogram), were stained with Human IL-5 Monoclonal Antibody (Catalog # MAB605) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG F(ab')₂ Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.