Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF652 - Provider product page
- Provider
- R&D Systems
- Product name
- Porcine IL-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-2 is observed.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P26891
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model.
Carter AJ, Aggarwal M, Kopia GA, Tio F, Tsao PS, Kolata R, Yeung AC, Llanos G, Dooley J, Falotico R
Cardiovascular research 2004 Sep 1;63(4):617-24
Cardiovascular research 2004 Sep 1;63(4):617-24
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-2 in Porcine PBMCs. IL-2 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Goat Anti-Porcine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF652) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-2 and Neutralization by Porcine IL-2 Antibody. Recombinant Porcine IL-2 (Catalog # 652-P2) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-2 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Porcine IL-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF652). The ND50 is typically 0.25-0.8 µg/mL.