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Western blot
ELISA
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-46957 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, less than 0.2% cross-reactivity with recombinant human IL-2 and recombinant mouse IL-2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-2 in monensin treated human peripheral blood mononuclear cells (PBMCs). Samples were incubated in IL-2 polyclonal antibody (Product # PA5-46957) using a dilution of 2 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. No additional treatment or additionally treated (+) with 0.5 µg/mL calcium ionomycin (Iono) and 50 ng/mL PMA overnight. A specific band was detected for IL‚2 at approximately.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IL-2 in immersion fixed rat splenocytes stimulated with calcium ionomycin and PMA. Samples were incubated in IL-2 polyclonal antibody (Product # PA5-46957) using a dilution of 5 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of IL-2 in immersion fixed rat splenocytes stimulated with calcium ionomycin and PMA. Samples were incubated in IL-2 polyclonal antibody (Product # PA5-46957) using a dilution of 5 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IL-2 in immersion fixed frozen sections of rat spleen. Samples were incubated in IL-2 polyclonal antibody (Product # PA5-46957) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL-2 in CTLL‚2 mouse cytotoxic T cell line. Samples were incubated in IL-2 polyclonal antibody (Product # PA5-46957). Recombinant Rat IL‚2 stimulates proliferation in the CTLL‚2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‚2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Rat IL‚2 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.15-0.75 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of rat IL-2 is shown by decrease in RFU (relative fluorescent units - measure of proliferation) with increasing concentrations of IL-2 polyclonal antibody (PA5-46957)
