NBP2-02441

antibody from Novus Biologicals

Targeting: PIM2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-02441

Provider

Novus Biologicals

Product name

Mouse Monoclonal PIM2 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.54 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PIM2 Antibody (5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIM2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human Kidney tissue using anti-PIM2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human liver tissue using anti-PIM2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human pancreas tissue using anti-PIM2 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody, and then analyzed by flow cytometry.