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antibody from Santa Cruz Biotechnology
Targeting: GNAS
GNAS1, GNASXL, GPSA, NESP, NESP55, SCG6, SgVI
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-823 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-823, RRID:AB_631538
- Product name
- Anti-GNAS
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references A novel alternatively spliced isoform of the mu-opioid receptor: functional antagonism.
Coordinate down-regulation of adenylyl cyclase isoforms and the stimulatory G protein (G(s)) in intestinal epithelial cell differentiation.
Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Activation of the progesterone-signaling pathway by methyl-beta-cyclodextrin or steroid in Xenopus laevis oocytes involves release of 45-kDa Galphas.
Alpha1-adrenergic receptors prevent a maladaptive cardiac response to pressure overload.
Gris P, Gauthier J, Cheng P, Gibson DG, Gris D, Laur O, Pierson J, Wentworth S, Nackley AG, Maixner W, Diatchenko L
Molecular pain 2010 Jun 2;6:33
Molecular pain 2010 Jun 2;6:33
Coordinate down-regulation of adenylyl cyclase isoforms and the stimulatory G protein (G(s)) in intestinal epithelial cell differentiation.
Choi LJ, Jenikova G, Hanson E, Spehlmann ME, Boehling NS, Kirstein SL, Bundey RA, Smith JR, Insel PA, Eckmann L
The Journal of biological chemistry 2010 Apr 23;285(17):12504-11
The Journal of biological chemistry 2010 Apr 23;285(17):12504-11
Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct.
Yu MJ, Pisitkun T, Wang G, Aranda JF, Gonzales PA, Tchapyjnikov D, Shen RF, Alonso MA, Knepper MA
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78
American journal of physiology. Cell physiology 2008 Sep;295(3):C661-78
Activation of the progesterone-signaling pathway by methyl-beta-cyclodextrin or steroid in Xenopus laevis oocytes involves release of 45-kDa Galphas.
Sadler SE, Archer MR, Spellman KM
Developmental biology 2008 Oct 1;322(1):199-207
Developmental biology 2008 Oct 1;322(1):199-207
Alpha1-adrenergic receptors prevent a maladaptive cardiac response to pressure overload.
O'Connell TD, Swigart PM, Rodrigo MC, Ishizaka S, Joho S, Turnbull L, Tecott LH, Baker AJ, Foster E, Grossman W, Simpson PC
The Journal of clinical investigation 2006 Apr;116(4):1005-15
The Journal of clinical investigation 2006 Apr;116(4):1005-15
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 47
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
