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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-8303 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8303, RRID:AB_649685
- Product name
- Anti-JAG1
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Epithelial Notch signaling regulates interstitial fibrosis development in the kidneys of mice and humans.
Expression of Notch pathway proteins correlates with albuminuria, glomerulosclerosis, and renal function
Regulation of notch1 signaling by nrf2: implications for tissue regeneration.
Inhibition of notch signaling blocks growth of glioblastoma cell lines and tumor neurospheres.
Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development.
Notch signaling is required for lateral induction of Jagged1 during FGF-induced lens fiber differentiation.
Sox2 and JAGGED1 expression in normal and drug-damaged adult mouse inner ear.
Bielesz B, Sirin Y, Si H, Niranjan T, Gruenwald A, Ahn S, Kato H, Pullman J, Gessler M, Haase VH, Susztak K
The Journal of clinical investigation 2010 Nov 1;120(11):4040-54
The Journal of clinical investigation 2010 Nov 1;120(11):4040-54
Expression of Notch pathway proteins correlates with albuminuria, glomerulosclerosis, and renal function
Mariana Murea, Jun-Ki Park, Shuchita Sharma, Hideki Kato, Antje Gruenwald, Thiruvur Niranjan, Han Si, David B Thomas, James M Pullman, Michal L Melamed, Katalin Susztak
Kidney International 2010 Jun;78(5):514-522
Kidney International 2010 Jun;78(5):514-522
Regulation of notch1 signaling by nrf2: implications for tissue regeneration.
Wakabayashi N, Shin S, Slocum SL, Agoston ES, Wakabayashi J, Kwak MK, Misra V, Biswal S, Yamamoto M, Kensler TW
Science signaling 2010 Jul 13;3(130):ra52
Science signaling 2010 Jul 13;3(130):ra52
Inhibition of notch signaling blocks growth of glioblastoma cell lines and tumor neurospheres.
Chen J, Kesari S, Rooney C, Strack PR, Chen J, Shen H, Wu L, Griffin JD
Genes & cancer 2010 Aug;1(8):822-35
Genes & cancer 2010 Aug;1(8):822-35
Murine Jagged1/Notch signaling in the second heart field orchestrates Fgf8 expression and tissue-tissue interactions during outflow tract development.
High FA, Jain R, Stoller JZ, Antonucci NB, Lu MM, Loomes KM, Kaestner KH, Pear WS, Epstein JA
The Journal of clinical investigation 2009 Jul;119(7):1986-96
The Journal of clinical investigation 2009 Jul;119(7):1986-96
Notch signaling is required for lateral induction of Jagged1 during FGF-induced lens fiber differentiation.
Saravanamuthu SS, Gao CY, Zelenka PS
Developmental biology 2009 Aug 1;332(1):166-76
Developmental biology 2009 Aug 1;332(1):166-76
Sox2 and JAGGED1 expression in normal and drug-damaged adult mouse inner ear.
Oesterle EC, Campbell S, Taylor RR, Forge A, Hume CR
Journal of the Association for Research in Otolaryngology : JARO 2008 Mar;9(1):65-89
Journal of the Association for Research in Otolaryngology : JARO 2008 Mar;9(1):65-89
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 134
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human gall bladder shows strong membranous and cytoplasmic positivity in glandular cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
