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Learn200-401-698S
antibody from Rockland Immunochemicals, Inc.
Targeting: JAG1
AGS, AHD, AWS, CD339, HJ1, JAGL1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 200-401-698S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-401-698S, RRID:AB_11182016
- Product name
- Anti-Jagged-1 (RABBIT) Antibody - 200-401-698S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
Submitted references Notch-Jagged signalling can give rise to clusters of cells exhibiting a hybrid epithelial/mesenchymal phenotype.
Boareto M, Jolly MK, Goldman A, Pietilä M, Mani SA, Sengupta S, Ben-Jacob E, Levine H, Onuchic JN
Journal of the Royal Society, Interface 2016 May;13(118)
Journal of the Royal Society, Interface 2016 May;13(118)
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Protein A purified anti-Jagged-1 antibody shows detection of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at ~134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.
- Validation comment
- Western Blot
