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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF599 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Jagged 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects rat Jagged 1 in ELISAs and Western blots. In sandwich ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Jagged 1 is observed, and less than 0.3% cross-reactivity with rhJagged 2 and recombinant mouse Jagged 2 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q63722- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration.
Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling.
PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia.
Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
Roles of Pofut1 and O-fucose in mammalian Notch signaling.
Wiszniak S, Schwarz Q
BMC neuroscience 2019 Apr 29;20(1):21
BMC neuroscience 2019 Apr 29;20(1):21
Muscle Satellite Cell Cross-Talk with a Vascular Niche Maintains Quiescence via VEGF and Notch Signaling.
Verma M, Asakura Y, Murakonda BSR, Pengo T, Latroche C, Chazaud B, McLoon LK, Asakura A
Cell stem cell 2018 Oct 4;23(4):530-543.e9
Cell stem cell 2018 Oct 4;23(4):530-543.e9
PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia.
Ola R, Dubrac A, Han J, Zhang F, Fang JS, Larrivée B, Lee M, Urarte AA, Kraehling JR, Genet G, Hirschi KK, Sessa WC, Canals FV, Graupera M, Yan M, Young LH, Oh PS, Eichmann A
Nature communications 2016 Nov 29;7:13650
Nature communications 2016 Nov 29;7:13650
Cadherin-based adhesions in the apical endfoot are required for active Notch signaling to control neurogenesis in vertebrates.
Hatakeyama J, Wakamatsu Y, Nagafuchi A, Kageyama R, Shigemoto R, Shimamura K
Development (Cambridge, England) 2014 Apr;141(8):1671-82
Development (Cambridge, England) 2014 Apr;141(8):1671-82
Roles of Pofut1 and O-fucose in mammalian Notch signaling.
Stahl M, Uemura K, Ge C, Shi S, Tashima Y, Stanley P
The Journal of biological chemistry 2008 May 16;283(20):13638-51
The Journal of biological chemistry 2008 May 16;283(20):13638-51
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Differentiation Induced by Jagged 1 and Neutralization by Rat Jagged 1 Antibody. Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND50 is typically 3-15 µg/mL.
