AP09127PU-N

antibody from Acris Antibodies GmbH

Targeting: JAG1 AGS, AHD, AWS, CD339, HJ1, JAGL1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09127PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09127PU-N, RRID:AB_2035312

Product name

anti CD339 / JAG1 (110-125)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 110-125 of Human Jagged-1 protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.5 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AP09127PU-N Jagged-1 antibody at 1/500 dilution shows detection of Jagged-1 protein in various whole cell lysates (20 µg/Lane). The membrane was washed and reacted with a 1/5,000 dilution of HRP conjugated Goat anti-Rabbit IgG. The band at ~134 kDa in Lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown.Lane 1: Human brain. Lane 2: Human kidney. Lane 3: Human liver. Lane 4: Sample buffer only.Lane 5: Mouse liver. Exposure time was 1 min.Predicted MW: 134 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence Microscopy using AP09127PU-N Jagged-1 antibody of Human corneal epithelial cells. Primary antibody was used at a 1/500 dilution. The Jagged-1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue). Personal Communication. Aihua Ma, Univdersity of Cardiff.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP09127PU-N Jagged-1 antibody Immunohistochemical staining of Formalin-Fixed, Paraffin Embedded Human cervical cancer tissue (40X magnification). Hematoxylin was used to counter-stain cells. A 1/100 dilution of primary antibody was used. Personal Communication. Martin Kast Laboratory.