AF1277

antibody from R&D Systems

Targeting: JAG1 AGS, AHD, AWS, CD339, HJ1, JAGL1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1277

Provider

R&D Systems

Product name

Human Jagged 1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Jagged 1 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant rat Jagged 1 and recombinant human Jagged 2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P78504


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

JAG1 overexpression contributes to Notch1 signaling and the migration of HTLV-1-transformed ATL cells.

Bellon M, Moles R, Chaib-Mezrag H, Pancewicz J, Nicot C
Journal of hematology & oncology 2018 Sep 19;11(1):119

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IL-4-dependent Jagged1 expression/processing is associated with survival of chronic lymphocytic leukemia cells but not with Notch activation.

De Falco F, Del Papa B, Baldoni S, Sabatini R, Falzetti F, Di Ianni M, Martelli MP, Mezzasoma F, Pelullo M, Marconi P, Sportoletti P, Screpanti I, Rosati E
Cell death & disease 2018 Nov 26;9(12):1160

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Evolution of Cancer Stem-like Cells in Endocrine-Resistant Metastatic Breast Cancers Is Mediated by Stromal Microvesicles.

Sansone P, Berishaj M, Rajasekhar VK, Ceccarelli C, Chang Q, Strillacci A, Savini C, Shapiro L, Bowman RL, Mastroleo C, De Carolis S, Daly L, Benito-Martin A, Perna F, Fabbri N, Healey JH, Spisni E, Cricca M, Lyden D, Bonafé M, Bromberg J
Cancer research 2017 Apr 15;77(8):1927-1941

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Interleukin-6 Stimulates Defective Angiogenesis.

Gopinathan G, Milagre C, Pearce OM, Reynolds LE, Hodivala-Dilke K, Leinster DA, Zhong H, Hollingsworth RE, Thompson R, Whiteford JR, Balkwill F
Cancer research 2015 Aug 1;75(15):3098-107

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A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.

Kulbe H, Chakravarty P, Leinster DA, Charles KA, Kwong J, Thompson RG, Coward JI, Schioppa T, Robinson SC, Gallagher WM, Galletta L, Australian Ovarian Cancer Study Group., Salako MA, Smyth JF, Hagemann T, Brennan DJ, Bowtell DD, Balkwill FR
Cancer research 2012 Jan 1;72(1):66-75

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Interleukin-6 as a therapeutic target in human ovarian cancer.

Coward J, Kulbe H, Chakravarty P, Leader D, Vassileva V, Leinster DA, Thompson R, Schioppa T, Nemeth J, Vermeulen J, Singh N, Avril N, Cummings J, Rexhepaj E, Jirström K, Gallagher WM, Brennan DJ, McNeish IA, Balkwill FR
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Sep 15;17(18):6083-96

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Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping.

Roesli C, Mumprecht V, Neri D, Detmar M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Jun;22(6):1933-44

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Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.

Liotta F, Angeli R, Cosmi L, Filì L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Jagged 1 by Western Blot. Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Jagged 1 in Human Kidney Cancer Tissue. Jagged 1 was detected in immersion fixed paraffin-embedded sections of human kidney cancer tissue using Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Alkaline Phosphatase Production Induced by Jagged 1 and Neutralization by Human Jagged 1 Antibody. Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Jagged 1 Antibody (Catalog # AF1277). The ND₅₀ is typically 1-5 µg/mL.