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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-585-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IFN-gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat and mouse IFN-gamma in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) IFN-gamma , recombinant canine IFN-gamma , recombinant feline IFN-gamma , recombinant equine IFN-gamma , recombinant porcine IFN-gamma , recombinant rhesus monkey IFN-gamma , recombinant cotton rat IFN-gamma , and recombinant bovine IFN-gamma is observed. In Western blots, less than 10% cross-reactivity with rhIFN-gamma is observed.
- Reactivity
- Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Expression of pro-inflammatory cytokines in the auditory cortex of rats with salicylate-induced tinnitus.
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.
Chen XH, Zheng LL
Molecular medicine reports 2017 Oct;16(4):5643-5648
Molecular medicine reports 2017 Oct;16(4):5643-5648
IFNβ Protects Neurons from Damage in a Murine Model of HIV-1 Associated Brain Injury.
Thaney VE, O'Neill AM, Hoefer MM, Maung R, Sanchez AB, Kaul M
Scientific reports 2017 Apr 20;7:46514
Scientific reports 2017 Apr 20;7:46514
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Shigemoto-Mogami Y, Hoshikawa K, Goldman JE, Sekino Y, Sato K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2231-43
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Feb 5;34(6):2231-43
Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.
Rana S, Byrne SN, MacDonald LJ, Chan CY, Halliday GM
The American journal of pathology 2008 Apr;172(4):993-1004
The American journal of pathology 2008 Apr;172(4):993-1004
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse/Rat IFN-gamma Antibody. Recombinant Rat IFN-gamma (Catalog # 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA). The ND50 is typically 0.08-0.2 µg/mL.
