Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB4851-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IFN-gamma Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IFN-gamma in direct ELISAs. Does not cross-react with rat, cotton rat, human, rhesus macaque, porcine, feline, or canine IFN-gamma .
- Reactivity
- Mouse
- Host
- Hamster
- Conjugate
- Unconjugated
- Antigen sequence
P01580
- Isotype
- IgG
- Antibody clone number
- H22
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Galectin-1-Driven Tolerogenic Programs Aggravate Yersinia enterocolitica Infection by Repressing Antibacterial Immunity.
The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
Lack of TNFR p55 results in heightened expression of IFN-γ and IL-17 during the development of reactive arthritis.
Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-γ, but not transforming growth factor-β dependent.
Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection.
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
Davicino RC, Méndez-Huergo SP, Eliçabe RJ, Stupirski JC, Autenrieth I, Di Genaro MS, Rabinovich GA
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1382-1392
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1382-1392
The encephalitogenicity of T(H)17 cells is dependent on IL-1- and IL-23-induced production of the cytokine GM-CSF.
El-Behi M, Ciric B, Dai H, Yan Y, Cullimore M, Safavi F, Zhang GX, Dittel BN, Rostami A
Nature immunology 2011 Jun;12(6):568-75
Nature immunology 2011 Jun;12(6):568-75
Lack of TNFR p55 results in heightened expression of IFN-γ and IL-17 during the development of reactive arthritis.
Eliçabe RJ, Cargnelutti E, Serer MI, Stege PW, Valdez SR, Toscano MA, Rabinovich GA, Di Genaro MS
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 1;185(7):4485-95
Journal of immunology (Baltimore, Md. : 1950) 2010 Oct 1;185(7):4485-95
Anti-Asthma Simplified Herbal Medicine Intervention-induced long-lasting tolerance to allergen exposure in an asthma model is interferon-γ, but not transforming growth factor-β dependent.
Srivastava K, Zhang T, Yang N, Sampson H, Li XM
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2010 Nov;40(11):1678-88
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2010 Nov;40(11):1678-88
Bystander central memory but not effector memory CD8+ T cells suppress allograft rejection.
Wan N, Dai H, Wang T, Moore Y, Zheng XX, Dai Z
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 1;180(1):113-21
Journal of immunology (Baltimore, Md. : 1950) 2008 Jan 1;180(1):113-21
In vivo blockade of OX40 ligand inhibits thymic stromal lymphopoietin driven atopic inflammation.
Seshasayee D, Lee WP, Zhou M, Shu J, Suto E, Zhang J, Diehl L, Austin CD, Meng YG, Tan M, Bullens SL, Seeber S, Fuentes ME, Labrijn AF, Graus YM, Miller LA, Schelegle ES, Hyde DM, Wu LC, Hymowitz SG, Martin F
The Journal of clinical investigation 2007 Dec;117(12):3868-78
The Journal of clinical investigation 2007 Dec;117(12):3868-78
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma in Mouse Splenocytes. IFN-gamma was detected in immersion fixed mouse splenocytes stimulated with calcium ionomycin and PMA (left panel) or unstimulated (right panel) using Hamster Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB4851) at 15 µg/mL for 3 hours at room temperature. Cells were stained using an anti-hamster IgG secondary antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IFN-gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN-gamma Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB4851). The ND50 is typically 4-20 ng/mL.