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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- AF5126 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal FRS2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat FRS2 isoforms when phosphorylated at Y436.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth.
Activation of the FGFR-STAT3 pathway in breast cancer cells induces a hyaluronan-rich microenvironment that licenses tumor formation.
Role of DNA methylation and epigenetic silencing of HAND2 in endometrial cancer development.
Msx homeobox genes critically regulate embryo implantation by controlling paracrine signaling between uterine stroma and epithelium.
The function of FGF signaling in the lens placode.
Xu L, Meng X, Xu N, Fu W, Tan H, Zhang L, Zhou Q, Qian J, Tu S, Li X, Lao Y, Xu H
Cell death & disease 2018 Feb 15;9(3):262
Cell death & disease 2018 Feb 15;9(3):262
Activation of the FGFR-STAT3 pathway in breast cancer cells induces a hyaluronan-rich microenvironment that licenses tumor formation.
Bohrer LR, Chuntova P, Bade LK, Beadnell TC, Leon RP, Brady NJ, Ryu Y, Goldberg JE, Schmechel SC, Koopmeiners JS, McCarthy JB, Schwertfeger KL
Cancer research 2014 Jan 1;74(1):374-86
Cancer research 2014 Jan 1;74(1):374-86
Role of DNA methylation and epigenetic silencing of HAND2 in endometrial cancer development.
Jones A, Teschendorff AE, Li Q, Hayward JD, Kannan A, Mould T, West J, Zikan M, Cibula D, Fiegl H, Lee SH, Wik E, Hadwin R, Arora R, Lemech C, Turunen H, Pakarinen P, Jacobs IJ, Salvesen HB, Bagchi MK, Bagchi IC, Widschwendter M
PLoS medicine 2013 Nov;10(11):e1001551
PLoS medicine 2013 Nov;10(11):e1001551
Msx homeobox genes critically regulate embryo implantation by controlling paracrine signaling between uterine stroma and epithelium.
Nallasamy S, Li Q, Bagchi MK, Bagchi IC
PLoS genetics 2012;8(2):e1002500
PLoS genetics 2012;8(2):e1002500
The function of FGF signaling in the lens placode.
Garcia CM, Huang J, Madakashira BP, Liu Y, Rajagopal R, Dattilo L, Robinson ML, Beebe DC
Developmental biology 2011 Mar 1;351(1):176-85
Developmental biology 2011 Mar 1;351(1):176-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 μM pervanadate (PV) for 10 minutes and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta-NGF (Catalog # 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
