66263-1-IG

antibody from Invitrogen Antibodies

Targeting: FRS2 FRS2A, FRS2alpha, SNT-1, SNT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

66263-1-IG

Provider

Invitrogen Antibodies

Product name

FRS2 Monoclonal Antibody (4E10H12)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: MGSCCSCPD KDTVPDNHRN KFKVINVDDD GNELGSGIME LTDTELILYT RKRDSVKWHY LCLRRYGYDS NLFSFESGRR CQTGQGIFAF KCARAEELFN MLQEIMQNNS INVVEEPVVE RNNHQTELEV PRTPRTPTTP GFAAQNLPNG YPRYPSFGDA SSHPSSRHPS VGSARLPSVG EESTHPLLVA EEQVHTYVNT TGVQEERKNR TSVHVPLEAR VSNAESSTPK EEPSSIEDRD PQILLEPEGV KFVLGPTPVQ KQLMEKEKLE QLGRDQVSGS GANNTEWDTG YDSDERRDAP S (1-300 aa encoded by BC021562)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4E10H12

Vial size

150 µL

Concentration

1.49 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF-7 cells were subjected to SDS PAGE followed by western blot with 66263-1-IG (FRS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using FRS2 antibody (Product # 66263-1-IG, Clone: 4E10H12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 66263-1-IG (FRS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66263-1-IG ( FRS2 Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 66263-1-IG ( FRS2 Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).