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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB6012 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6012, RRID:AB_1580887
- Product name
- SOCS1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of SOCS1.
- Antigen sequence
C-VLRDYLSSFPFQI- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references A family of cytokine-inducible inhibitors of signalling.
Starr R, Willson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ
Nature 1997 Jun 26;387(6636):917-21
Nature 1997 Jun 26;387(6636):917-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SOCS1 polyclonal antibody (Cat # PAB6012) staining (2 ug/mL) of mouse spleen lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing SOCS1 and probed with SOCS1 polyclonal antibody (Cat # PAB6012) (mock transfection in lane 2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SOCS1 polyclonal antibody (Cat # PAB6012, 2.5 ug/mL) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
