PA5-17219
antibody from Invitrogen Antibodies
Targeting: SOCS2
CIS2, Cish2, SOCS-2, SSI-2, SSI2, STATI2
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-17219 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SOCS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 374 µg/mL
- Storage
- -20°C
Submitted references Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages.
Yin G, Lin Y, Wang P, Zhou J, Lin H
Molecular medicine (Cambridge, Mass.) 2022 Jun 29;28(1):76
Molecular medicine (Cambridge, Mass.) 2022 Jun 29;28(1):76
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SOCS2 in extracts from A204 and PC12 cells using SOCS2 polyclonal antibody (Product # PA5-17219).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SOCS2 was achieved by transfecting Caco-2 with SOCS2 specific siRNAs (Silencer® select Product # s16860, s16859). Western blot analysis (Fig. a) was performed using whole cell extracts from the SOCS2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SOCS2 Polyclonal Antibody (Product # PA5-17219, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SOCS2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SOCS2 Polyclonal Antibody (Product # PA5-17219) and a 22kDa band corresponding to SOCS2 was observed across cell lines tested except U-2 OS and Jurkat which are reported to be negative for SOCS2 expression. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), Caco-2 (Lane 2), PC-3 (Lane 3), HCT 116 (Lane 4), U-2 OS (Lane 5) and Jurkat (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).