sc-903

antibody from Santa Cruz Biotechnology

Targeting: PRKACA PKACa

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-903

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-903, RRID:AB_2268772

Product name

Anti-PRKACA

Antibody type

Polyclonal (Antigen purified)

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

References

Submitted references

Serine residue 115 of MAPK-activated protein kinase MK5 is crucial for its PKA-regulated nuclear export and biological function.

Kostenko S, Shiryaev A, Gerits N, Dumitriu G, Klenow H, Johannessen M, Moens U
Cellular and molecular life sciences : CMLS 2011 Mar;68(5):847-62

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Protein kinase A type I activates a CRE-element more efficiently than protein kinase A type II regardless of C subunit isoform.

Stakkestad Ø, Larsen AC, Kvissel AK, Eikvar S, Ørstavik S, Skålhegg BS
BMC biochemistry 2011 Feb 8;12:7

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Protein kinase A phosphorylation activates Vpr-induced cell cycle arrest during human immunodeficiency virus type 1 infection.

Barnitz RA, Wan F, Tripuraneni V, Bolton DL, Lenardo MJ
Journal of virology 2010 Jul;84(13):6410-24

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Protein kinase A regulatory subunit distribution in medulloblastoma.

Mucignat-Caretta C, Denaro L, Redaelli M, D'Avella D, Caretta A
BMC cancer 2010 Apr 14;10:141

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Identification of novel in vivo phosphorylation sites of the human proapoptotic protein BAD: pore-forming activity of BAD is regulated by phosphorylation.

Polzien L, Baljuls A, Rennefahrt UEE, Fischer A, Schmitz W, Zahedi RP, Sickmann A, Metz R, Albert S, Benz R, Hekman M, Rapp UR
The Journal of biological chemistry 2009 Oct 9;284(41):28004-28020

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The PKs PKA and ERK 1/2 are involved in phosphorylation of TH at Serine 40 and 31 during morphine withdrawal in rat hearts.

Almela P, Milanés M, Laorden M
British journal of pharmacology 2008 Sep;155(1):73-83

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Different phases of long-term memory require distinct temporal patterns of PKA activity after single-trial classical conditioning.

Michel M, Kemenes I, Müller U, Kemenes G
Learning & memory (Cold Spring Harbor, N.Y.) 2008 Sep;15(9):694-702

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14-3-3 theta binding to cell cycle regulatory factors is enhanced by HIV-1 Vpr.

Bolton DL, Barnitz RA, Sakai K, Lenardo MJ
Biology direct 2008 Apr 29;3:17

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Renal activation of extracellular signal-regulated kinase in rats with autosomal-dominant polycystic kidney disease

Shizuko Nagao, Tamio Yamaguchi, Masatomo Kusaka, Robin L Maser, Hisahide Takahashi, Benjamin D Cowley, Jared J Grantham
Kidney International 2003 Feb;63(2):427-437

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cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein.

Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB
Proceedings of the National Academy of Sciences of the United States of America 2002 Dec 24;99(26):16800-5

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 40

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.