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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB4529 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4529, RRID:AB_1579115
- Product name
- PRKACA polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKACA.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Active cAMP-dependent protein kinase incorporated within highly purified HIV-1 particles is required for viral infectivity and interacts with viral capsid protein.
Modulation of cardiac sodium channel gating by protein kinase A can be altered by disease-linked mutation.
Differential regulation of P-selectin expression by protein kinase A and protein kinase G in thrombin-stimulated human platelets.
Cartier C, Hemonnot B, Gay B, Bardy M, Sanchiz C, Devaux C, Briant L
The Journal of biological chemistry 2003 Sep 12;278(37):35211-9
The Journal of biological chemistry 2003 Sep 12;278(37):35211-9
Modulation of cardiac sodium channel gating by protein kinase A can be altered by disease-linked mutation.
Tateyama M, Rivolta I, Clancy CE, Kass RS
The Journal of biological chemistry 2003 Nov 21;278(47):46718-26
The Journal of biological chemistry 2003 Nov 21;278(47):46718-26
Differential regulation of P-selectin expression by protein kinase A and protein kinase G in thrombin-stimulated human platelets.
Libersan D, Rousseau G, Merhi Y
Thrombosis and haemostasis 2003 Feb;89(2):310-7
Thrombosis and haemostasis 2003 Feb;89(2):310-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PRKACA polyclonal antibody (Cat # PAB4529) is used in Western blot to detect PRKACA alpha in Jurkat cell lysate .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PRKACA polyclonal antibody (Cat # PAB4529) is used in Western blot to detect PRKACA alpha in mouse heart tissue lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PRKACA polyclonal antibody (Cat # PAB4529) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . HC = hepatocarcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
