Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-17626 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKA alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 56 µg/mL
- Storage
- -20°C
Submitted references Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.
Long X, Wu H, Zhou Y, Wan Y, Kan X, Gong J, Zhao X
Frontiers in nutrition 2022;9:852012
Frontiers in nutrition 2022;9:852012
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PRKACA was achieved by transfecting NIH: OVCAR-3 with PRKACA specific siRNAs (Silencer® select Product # s8587, s8586). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the PRKACA knockdown cells (Lane 1), non-specific scrambled siRNA transfected cells (Lane 1) and untransfected cells (Lane 3). The blot was probed with PKA alpha Polyclonal Antibody (Product # PA5-17626, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown i histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PRKACA.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-PKA alpha Polyclonal Antibody (Product # PA5-17626) and a 40 kDa band corresponding to PRKACA was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of NIH: OVCAR-3 (Lane 1), A549 (Lane 2), Jurkat (Lane 3), NIH:3T3 (Lane 4), C2C12 (Lane 5), HL-60 (Lane 6), HeLa (Lane 7), Mouse lung (Lane 8), Rat lung (Lane 9), Mouse kidney (Lane 10), Rat kidney (Lane 11) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PKA C-alpha in HeLa cells using a PKA C-alpha polyclonal antibody (Product # PA5-17626) (green). Actin filaments are labeled with a fluorescent red phalloidin. DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PKA C-alpha in HeLa cells using a PKA C-alpha polyclonal antibody (Product # PA5-17626) (blue) compared to a nonspecific negative control antibody (red).