NB300-613

antibody from Novus Biologicals

Targeting: CLTC CLTCL2, Hc

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB300-613

Provider

Novus Biologicals

Proper citation

Novus Cat#NB300-613, RRID:AB_10130252

Product name

Mouse Monoclonal Clathrin Heavy Chain 1/CHC17 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Detects clathrin heavy chain.

Reactivity

Human, Mouse, Rat, Bovine, Hamster, Simian

Host

Mouse

Antigen sequence

Electron microscopy and proteolysis
mapping demonstrate that binding o
ccurs towards the central hub of th
e triskelion, N-terminal to the lig
ht chain binding regions.


Isotype

IgG

Vial size

100uL

Concentration

6 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Quantification of the Relative Contributions of Loss-of-function and Gain-of-function Mechanisms in TAR DNA-binding Protein 43 (TDP-43) Proteinopathies.

Cascella R, Capitini C, Fani G, Dobson CM, Cecchi C, Chiti F
The Journal of biological chemistry 2016 Sep 9;291(37):19437-48

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Varying label density allows artifact-free analysis of membrane-protein nanoclusters.

Baumgart F, Arnold AM, Leskovar K, Staszek K, Fölser M, Weghuber J, Stockinger H, Schütz GJ
Nature methods 2016 Aug;13(8):661-4

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Dab2 inhibits the cholesterol-dependent activation of JNK by TGF-β.

Shapira KE, Hirschhorn T, Barzilay L, Smorodinsky NI, Henis YI, Ehrlich M
Molecular biology of the cell 2014 May;25(10):1620-8

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Differential regulation of Smad3 and of the type II transforming growth factor-β receptor in mitosis: implications for signaling.

Hirschhorn T, Barizilay L, Smorodinsky NI, Ehrlich M
PloS one 2012;7(8):e43459

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Lysine acetylation induced by chronic ethanol consumption impairs dynamin-mediated clathrin-coated vesicle release.

Shepard BD, Tuma DJ, Tuma PL
Hepatology (Baltimore, Md.) 2012 Apr;55(4):1260-70

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HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits.

Gottfried I, Ehrlich M, Ashery U
Cellular and molecular life sciences : CMLS 2009 Sep;66(17):2897-911

---

Ethanol selectively impairs clathrin-mediated internalization in polarized hepatic cells.

Fernandez DJ, McVicker BL, Tuma DJ, Tuma PL
Biochemical pharmacology 2009 Sep 15;78(6):648-55

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of 25 ug of human brain lysates.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Cancer biopsies of deparaffinized Human breast carcinoma tissue.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Normal biopsies of deparaffinized Human colon tissue.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Clathrin Heavy Chain 1/CHC17 Antibody (X22) [NB300-613] - Analysis of Alexa Fluor (R) 488 conjugate of NB300-613. A cell surface stain was performed on HeLa cells with Clathrin Heavy Chain 1 antibody NB300-613AF488 (blue) along with a matched isotype control NBP2-27287AF488 (orange). Cells were incubated in an a