H00001213-M05

antibody from Abnova Corporation

Targeting: CLTC CLTCL2, Hc

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001213-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001213-M05, RRID:AB_581671

Product name

CLTC monoclonal antibody (M05), clone 2E5

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant CLTC.

Antigen sequence

EVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQI
SEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGE
TIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIP
YITN


Isotype

IgG

Antibody clone number

2E5

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CLTC is 0.3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between HIP1 and CLTC. HeLa cells were stained with anti-HIP1 rabbit purified polyclonal 1:1200 and anti-CLTC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)