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Western blot
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- Antibody Data
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- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
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- Product number
- SM5011P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#SM5011P, RRID:AB_978084
- Product name
- anti Clathrin heavy chain
- Antibody type
- Monoclonal
- Antigen
- Purified Human brain clathrin heavy chain.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- X22
- Vial size
- 0.1 ml
- Concentration
- 6.0 mg/ml
Submitted references Flat clathrin coats on endosomes mediate degradative protein sorting by scaffolding Hrs in dynamic microdomains.
Raiborg C, Wesche J, Malerød L, Stenmark H
Journal of cell science 2006 Jun 15;119(Pt 12):2414-24
Journal of cell science 2006 Jun 15;119(Pt 12):2414-24
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Clathrin, Heavy chain was performed by loading 25 ug of human brain lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Clathrin, Heavy chain monoclonal antibody (Cat.-No SM5011P) at a dilution of 1:300 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG + IgM (H+L) cross-adsorbed secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate. Results show a band at ~180 kDa.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) (Cat.-No SM5011P) shows staining in U251 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain (Cat.-No SM5011P) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Goat Anti-Mouse). Images were taken at 60X magnification.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) (Cat.-No SM5011P) shows staining in NCI-H460 Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain ((Cat.-No SM5011P) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Goat Anti-Mouse). Images were taken at 60X magnification.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Clathrin, Heavy chain using Anti-Clathrin, Heavy chain Monoclonal Antibody (X22) (Cat.-No SM5011P) shows staining in Hela Cells. Clathrin, Heavy chain staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Clathrin, Heavy chain (Cat.-No SM5011P) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Goat Anti-Mouse). Images were taken at 60X magnification.
