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- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- AF692 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse RANK/TNFRSF11A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse RANK/TNFRSF11A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human RANK is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O35305- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis.
Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation.
Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.
Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.
MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunity.
Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors.
The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
Jhun J, Lee SH, Kim SY, Ryu J, Kwon JY, Na HS, Jung K, Moon SJ, Cho ML, Min JK
Journal of translational medicine 2019 Mar 15;17(1):84
Journal of translational medicine 2019 Mar 15;17(1):84
Endothelial proteolytic activity and interaction with non-resorbing osteoclasts mediate bone elongation.
Romeo SG, Alawi KM, Rodrigues J, Singh A, Kusumbe AP, Ramasamy SK
Nature cell biology 2019 Apr;21(4):430-441
Nature cell biology 2019 Apr;21(4):430-441
Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6.
Danzl NM, Jeong S, Choi Y, Alexandropoulos K
PloS one 2014;9(1):e86129
PloS one 2014;9(1):e86129
Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V
Autophagy 2014;10(11):2036-52
Autophagy 2014;10(11):2036-52
OPG/RANKL/RANK axis is a critical inflammatory signaling system in ischemic brain in mice.
Shimamura M, Nakagami H, Osako MK, Kurinami H, Koriyama H, Zhengda P, Tomioka H, Tenma A, Wakayama K, Morishita R
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 3;111(22):8191-6
Proceedings of the National Academy of Sciences of the United States of America 2014 Jun 3;111(22):8191-6
MHC class II transactivator is an in vivo regulator of osteoclast differentiation and bone homeostasis co-opted from adaptive immunity.
Benasciutti E, Mariani E, Oliva L, Scolari M, Perilli E, Barras E, Milan E, Orfanelli U, Fazzalari NL, Campana L, Capobianco A, Otten L, Particelli F, Acha-Orbea H, Baruffaldi F, Faccio R, Sitia R, Reith W, Cenci S
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2014 Feb;29(2):290-303
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2014 Feb;29(2):290-303
Generation of both cortical and Aire(+) medullary thymic epithelial compartments from CD205(+) progenitors.
Baik S, Jenkinson EJ, Lane PJ, Anderson G, Jenkinson WE
European journal of immunology 2013 Mar;43(3):589-94
European journal of immunology 2013 Mar;43(3):589-94
The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP
Developmental biology 2009 Apr 1;328(1):127-39
Developmental biology 2009 Apr 1;328(1):127-39
The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
Kovacić N, Lukić IK, Grcević D, Katavić V, Croucher P, Marusić A
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3379-89
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3379-89
The Fas/Fas ligand system inhibits differentiation of murine osteoblasts but has a limited role in osteoblast and osteoclast apoptosis.
Kovacić N, Lukić IK, Grcević D, Katavić V, Croucher P, Marusić A
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3379-89
Journal of immunology (Baltimore, Md. : 1950) 2007 Mar 15;178(6):3379-89
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- RANK/TNFRSF11A in Rat Embryo. RANK/TNFRSF11A was detected in immersion fixed frozen sections of rat embryo (cartillage primordium, 7 days p.c.) using 10 µg/mL Goat Anti-Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF692) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Mouse RANK/TNFRSF11A Antibody Induces Osteoclast Differentiation in Mouse Splenocytes. Mouse RANK/TNFRSF11A Antigen Affinity-purified Polyclonal Antibody induces osteoclast differentiation on mouse splenocytes in the presence of 20 ng/mL recombinant mouse M-CSF (Catalog # 416-ML), in a dose-dependent manner, as measured by the tartrate-resistant acid phosphatase (TRAP) solution assay. The ED50 for this effect is typically 0.1-0.4 µg/mL.
