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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Flow cytometry [4]
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Validation data
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- Product number
- MA5-16153 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RANK Monoclonal Antibody (9A725)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: Raw.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9A725
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibition of Osteoclastogenesis by the RNA-Binding Protein QKI5: a Novel Approach to Protect from Bone Resorption.
Chondrocytes-Specific Expression of Osteoprotegerin Modulates Osteoclast Formation in Metaphyseal Bone.
Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
Rauwel B, Degboé Y, Diallo K, Sayegh S, Baron M, Boyer JF, Constantin A, Cantagrel A, Davignon JL
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2020 Apr;35(4):753-765
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2020 Apr;35(4):753-765
Chondrocytes-Specific Expression of Osteoprotegerin Modulates Osteoclast Formation in Metaphyseal Bone.
Wang B, Jin H, Shu B, Mira RR, Chen D
Scientific reports 2015 Sep 2;5:13667
Scientific reports 2015 Sep 2;5:13667
Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
Riegel A, Maurer T, Prior B, Stegmaier S, Heppert V, Wagner C, Hänsch GM
European journal of immunology 2012 Apr;42(4):975-81
European journal of immunology 2012 Apr;42(4):975-81
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RANK in transfected 293 cells using a CD265/RANK monoclonal antibody (Product # MA5-16153) at 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RANK in transfected 293 cells. Samples were incubated in RANK monoclonal antibody (Product # MA5-16153) using a dilution of 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RANK in K-562 cells (blue) and a matched isotype control (orange). Samples were incubated in RANK monoclonal antibody (Product # MA5-16153) using a dilution of 1 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RANK in Raw 264.7 cells. Samples were incubated in RANK monoclonal antibody (Product # MA5-16153) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RANK in 10^6 RAW cells. Samples were incubated in RANK monoclonal antibody (Product # MA5-16153) using a dilution of 2 µg. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of RANK in RAW cells. Samples were incubated in RANK monoclonal antibody (Product # MA5-16153) using a dilution of 0.5 µg. Green histogram represents isotype control; red represents RANK antibody (PE conjugated).
