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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
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- Validations
- Western blot [1]
- Flow cytometry [4]
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- Product number
- NB100-56508 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56508, RRID:AB_838765
- Product name
- Mouse Monoclonal RANK/TNFRSF11A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In RAW cells, a 97 kDa is observed.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Osteoblasts contribute to a protective niche that supports melanoma cell proliferation and survival.
Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails.
RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients.
Characterization of Regulatory Extracellular Vesicles from Osteoclasts.
Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice.
Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G₁ phase and protects them from UV light-induced apoptosis.
Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats.
RANK receptor oligomerisation in the regulation of NFκB signalling.
c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton.
Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples.
Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation.
Follicle-stimulating hormone promotes RANK expression on human monocytes.
Signal peptide mutations in RANK prevent downstream activation of NF-κB.
Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta.
Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Ferguson J, Wilcock DJ, McEntegart S, Badrock AP, Levesque M, Dummer R, Wellbrock C, Smith MP
Pigment cell & melanoma research 2020 Jan;33(1):74-85
Pigment cell & melanoma research 2020 Jan;33(1):74-85
Osteoclastogenesis from bone marrow cells during estrogen-induced medullary bone formation in Japanese quails.
Hiyama S, Yokoi M, Akagi Y, Kadoyama Y, Nakamori K, Tsuga K, Uchida T, Terayama R
Journal of molecular histology 2019 Aug;50(4):389-404
Journal of molecular histology 2019 Aug;50(4):389-404
RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients.
Nanke Y, Kobashigawa T, Yago T, Kawamoto M, Yamanaka H, Kotake S
BioMed research international 2016;2016:4874195
BioMed research international 2016;2016:4874195
Characterization of Regulatory Extracellular Vesicles from Osteoclasts.
Huynh N, VonMoss L, Smith D, Rahman I, Felemban MF, Zuo J, Rody WJ Jr, McHugh KP, Holliday LS
Journal of dental research 2016 Jun;95(6):673-9
Journal of dental research 2016 Jun;95(6):673-9
Impaired differentiation of macrophage lineage cells attenuates bone remodeling and inflammatory angiogenesis in Ndrg1 deficient mice.
Watari K, Shibata T, Nabeshima H, Shinoda A, Fukunaga Y, Kawahara A, Karasuyama K, Fukushi J, Iwamoto Y, Kuwano M, Ono M
Scientific reports 2016 Jan 18;6:19470
Scientific reports 2016 Jan 18;6:19470
Lipopolysaccharide treatment arrests the cell cycle of BV-2 microglial cells in G₁ phase and protects them from UV light-induced apoptosis.
Kaneko YS, Ota A, Nakashima A, Nagasaki H, Kodani Y, Mori K, Nagatsu T
Journal of neural transmission (Vienna, Austria : 1996) 2015 Feb;122(2):187-99
Journal of neural transmission (Vienna, Austria : 1996) 2015 Feb;122(2):187-99
Elevated suppressor of cytokine signaling-1 (SOCS-1): a mechanism for dysregulated osteoclastogenesis in HIV transgenic rats.
Lafferty MK, Fantry L, Bryant J, Jones O, Hammoud D, Weitzmann MN, Lewis GK, Garzino-Demo A, Reid W
Pathogens and disease 2014 Jun;71(1):81-9
Pathogens and disease 2014 Jun;71(1):81-9
RANK receptor oligomerisation in the regulation of NFκB signalling.
Das S, Sepahi I, Duthie A, Clark S, Crockett JC
Journal of molecular endocrinology 2014 Aug;53(1):81-91
Journal of molecular endocrinology 2014 Aug;53(1):81-91
c-Src links a RANK/αvβ3 integrin complex to the osteoclast cytoskeleton.
Izawa T, Zou W, Chappel JC, Ashley JW, Feng X, Teitelbaum SL
Molecular and cellular biology 2012 Jul;32(14):2943-53
Molecular and cellular biology 2012 Jul;32(14):2943-53
Human polymorphonuclear neutrophils express RANK and are activated by its ligand, RANKL.
Riegel A, Maurer T, Prior B, Stegmaier S, Heppert V, Wagner C, Hänsch GM
European journal of immunology 2012 Apr;42(4):975-81
European journal of immunology 2012 Apr;42(4):975-81
An efficient and rapid method for cDNA cloning from difficult templates using codon optimization and SOE-PCR: with human RANK and TIMP2 gene as examples.
Huang G, Wen Q, Gao Q, Zhang F, Bai Y
Biotechnology letters 2011 Oct;33(10):1939-47
Biotechnology letters 2011 Oct;33(10):1939-47
Locally administered interferon-γ accelerates lipopolysaccharide-induced osteoclastogenesis independent of immunohistological RANKL upregulation.
Ayon Haro ER, Ukai T, Yokoyama M, Kishimoto T, Yoshinaga Y, Hara Y
Journal of periodontal research 2011 Jun;46(3):361-73
Journal of periodontal research 2011 Jun;46(3):361-73
Follicle-stimulating hormone promotes RANK expression on human monocytes.
Cannon JG, Kraj B, Sloan G
Cytokine 2011 Feb;53(2):141-4
Cytokine 2011 Feb;53(2):141-4
Signal peptide mutations in RANK prevent downstream activation of NF-κB.
Crockett JC, Mellis DJ, Shennan KI, Duthie A, Greenhorn J, Wilkinson DI, Ralston SH, Helfrich MH, Rogers MJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Aug;26(8):1926-38
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Aug;26(8):1926-38
Direct inhibition of human RANK+ osteoclast precursors identifies a homeostatic function of IL-1beta.
Lee B, Kim TH, Jun JB, Yoo DH, Woo JH, Choi SJ, Lee YH, Song GG, Sohn J, Park-Min KH, Ivashkiv LB, Ji JD
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):5926-34
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):5926-34
Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism.
Bharti AC, Takada Y, Shishodia S, Aggarwal BB
The Journal of biological chemistry 2004 Feb 13;279(7):6065-76
The Journal of biological chemistry 2004 Feb 13;279(7):6065-76
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90
Blood 2001 Nov 1;98(9):2784-90
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines.
Fiumara P, Snell V, Li Y, Mukhopadhyay A, Younes M, Gillenwater AM, Cabanillas F, Aggarwal BB, Younes A
Blood 2001 Nov 1;98(9):2784-90
Blood 2001 Nov 1;98(9):2784-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis of RANK in transfected 293 cells using RANK antibody at 2 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Intracellular flow cytometry analysis of RANK in 10^6 RAW cells using 2 ug of RANK antibody. Shaded histogram represents RAW cells without antibody; green represents isotype control; antibody red represents RANK antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Analysis using the PE conjugate of NB100-56508. Staining of RANK in RAW cells using 0.5 ug of RANK antibody. Green histogram represents isotype control; red represents RANK antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - Using the PE direct conjugate An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [NB100-56508] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
