PAB2980

antibody from Abnova Corporation

Targeting: RASGRF1 CDC25, CDC25L, GNRP, GRF1, GRF55, H-GRF55, PP13187

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2980

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2980, RRID:AB_1579587

Product name

RASGRF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RASGRF1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Zoledronate-induced S phase arrest and apoptosis accompanied by DNA damage and activation of the ATM/Chk1/cdc25 pathway in human osteosarcoma cells.

Iguchi T, Miyakawa Y, Saito K, Nakabayashi C, Nakanishi M, Saya H, Ikeda Y, Kizaki M
International journal of oncology 2007 Aug;31(2):285-91

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Timing and sequence requirements defined for embryonic maintenance of imprinted DNA methylation at Rasgrf1.

Holmes R, Chang Y, Soloway PD
Molecular and cellular biology 2006 Dec;26(24):9564-70

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MDA-MB-231 cell lysate (35 ug/lane) with RASGRF1 polyclonal antibody (Cat # PAB2980).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human brain tissue reacted with RASGRF1 polyclonal antibody (Cat # PAB2980) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)