- PA5-31773 - Invitrogen Antibodies KIF23 antibody

Gibieža P, Peterman E, Hoffman HK, Van Engeleburg S, Skeberdis VA, Prekeris R
Molecular biology of the cell 2021 Apr 1;32(7):554-566

Wu H, Tian X, Zhu C
Cancer cell international 2020;20:283

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of MKLP1 using 30 µg of HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a MKLP1 polyclonal antibody (Product # PA5-31773) at a dilution of 1:2000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot using KIF23 Polyclonal Antibody (Product # PA5-31773). Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with KIF23 Polyclonal Antibody (Product # PA5-31773) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry-Immunofluorescence analysis of KIF23 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: KIF23 Polyclonal Antibody (Product # PA5-31773) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: KIF23 Polyclonal Antibody detects MKLP1 protein at midbody by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by KIF23 Polyclonal Antibody (Product # PA5-31773) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 4 KIF23 was up-regulated in PCa tissues and cell lines and targeted by miR-15a-5p. a , b The expression of KIF23 at mRNA and protein levels in PCa tissues and normal tissues was measured by qRT-PCR and western blot, respectively. c , d The expression of KIF23 at mRNA and protein levels in PCa cells and normal cells was measured by qRT-PCR and western blot, respectively. e The expression of miR-15a-5p was negatively correlated with KIF23 expression. f The binding sites between miR-15a-5p and KIF23 3' UTR were forecasted by starBase v2.0. g The interaction between miR-15a-5p and KIF23 was confirmed by dual-luciferase reporter assay. h , i The expression of KIF23 was modulated by miR-15a-5p expression at both mRNA and protein levels. * P < 0.05, ** P < 0.01, *** P < 0.001

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 7 Knockdown of PVT1 impeded the PCa progression in vivo. a The tumor volume was calculated every 4 day after 10 day-inoculation. b The tumor weight was measured after 34 days. c The expression of PVT1, miR-15a-5p and KIF23, d the expression of KIF23 at the protein levels, e and the levels of Vimentin, E-cad, Caspase-3 and CyclinD1 were examined in removed tumor tissues using qRT-PCR or western blot. * P < 0.05, ** P < 0.01

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Additional file 3: Figure S2. KIF23 overexpression rescued the effects of PVT1 knockdown. 22RV1 and DU145 cells were transfected with si-PVT1 or si-VT1 + oe-KIF23, with si-NC or or si-PVT1 + vector as the control. (A andB) The expression of KIF23 in these transfected cells was detected by qRT-PCR an western blot. (C) Cell proliferation was assessed by MTT assay. (D) Cell apoptosis was monitored by flow cytometry assay. (E and F) Cell migration and cell invasion in these transfected cells were investigated by transwell assay. (G) The expression of Vimentin, E-cad, Caspase-3 and CyclinD1 was quantified by western blot in these transfected cells. * P < 0.05, ** P < 0.01.

PA5-31773