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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PA5-87749 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TIAM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SILRDKHRRQ LLKTESLPSS QQYVPFGGKR LCALKGARPA MSRAVSAPSK SLGRRRRRLA RNRFTIDSDA VSASSPEKES QQPPGGGDTD RWVEEQFDLA QYEEQDDIKE TDILSDDDEF CESVKGASVD RDLQERLQAT SISQRERGRK TLDSHASRMA QLKKQAALSG INGGLESASE EVIWVRREDF APSRKLNTEI; Positive Samples: B cells, Jurkat, A-431, U-87MG, SW480; Cellular Location: Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.51 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using TIAM1 Polyclonal antibody (Product # PA5-87749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TIAM1 in paraffin-embedded human lung cancer using TIAM1 Polyclonal Antibody (Product # PA5-87749) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TIAM1 in paraffin-embedded human colon carcinoma using TIAM1 Polyclonal Antibody (Product # PA5-87749) at a dilution of 1:100.
