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Learn12530-1-AP
antibody from Invitrogen Antibodies
Targeting: RRAS2
TC21
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 12530-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RRAS2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAAGWRDG SGQEKYRLVV VGGGGVGKSA LTIQFIQSYF VTDYDPTIED SYTKQCVIDD RAARLDILDT AGQEEFGAMR EQYMRTGEGF LLVFSVTDRG SFEEIYKFQR QILRVKDRDE FPMILIGNKA DLDHQRQVTQ EEGQQLARQL KVTYMEASAK IRMNVDQAFH ELVRVIRKFQ EQECPPSPEP TRKEKDKKGC HCVIF (1-204 aa encoded by BC013106)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12530-1-AP (RRAS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A431 cells using 12530-1-AP (RRAS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart tissue slide using 12530-1-AP (RRAS2 Antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 12530-1-AP (RRAS2 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12530-1-AP ( RRAS2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12530-1-AP ( RRAS2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 A431 cells were stained with .2ug RRAS2 antibody (12530-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RRAS2 (IP:12530-1-AP, 4ug; Detection:12530-1-AP 1:800) with NIH/3T3 cells lysate 4000ug.
