AF2538

antibody from Novus Biologicals

Targeting: DDR2 NTRKR3, TKT, TYRO10

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF2538

Provider

Novus Biologicals

Product name

Goat Polyclonal DDR2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human DDR2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human DDR1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Chain alignment of collagen I deciphered using computationally designed heterotrimers.

Jalan AA, Sammon D, Hartgerink JD, Brear P, Stott K, Hamaia SW, Hunter EJ, Walker DR, Leitinger B, Farndale RW
Nature chemical biology 2020 Apr;16(4):423-429

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Discoidin domain receptor 2 mediates collagen-induced activation of membrane-type 1 matrix metalloproteinase in human fibroblasts.

Majkowska I, Shitomi Y, Ito N, Gray NS, Itoh Y
The Journal of biological chemistry 2017 Apr 21;292(16):6633-6643

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Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.

Iwai LK, Payne LS, Luczynski MT, Chang F, Xu H, Clinton RW, Paul A, Esposito EA, Gridley S, Leitinger B, Naegle KM, Huang PH
The Biochemical journal 2013 Sep 15;454(3):501-13

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Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice.

Kawai I, Hisaki T, Sugiura K, Naito K, Kano K
Biochemical and biophysical research communications 2012 Oct 26;427(3):611-7

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DDR2 plays a role in fibroblast migration independent of adhesion ligand and collagen activated DDR2 tyrosine kinase.

Herrera-Herrera ML, Quezada-Calvillo R
Biochemical and biophysical research communications 2012 Dec 7;429(1-2):39-44

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Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1.

Xu H, Raynal N, Stathopoulos S, Myllyharju J, Farndale RW, Leitinger B
Matrix biology : journal of the International Society for Matrix Biology 2011 Jan;30(1):16-26

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human DDR2 by Simple Western^TM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with Calyculin A for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for DDR2 at approximately 139 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human DDR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2538) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.