Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF2538 - Provider product page
- Provider
- R&D Systems
- Product name
- Human DDR2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human DDR2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human DDR1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q16832
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Discoidin domain receptor 2 mediates collagen-induced activation of membrane-type 1 matrix metalloproteinase in human fibroblasts.
Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice.
DDR2 plays a role in fibroblast migration independent of adhesion ligand and collagen activated DDR2 tyrosine kinase.
Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1.
Majkowska I, Shitomi Y, Ito N, Gray NS, Itoh Y
The Journal of biological chemistry 2017 Apr 21;292(16):6633-6643
The Journal of biological chemistry 2017 Apr 21;292(16):6633-6643
Phosphoproteomics of collagen receptor networks reveals SHP-2 phosphorylation downstream of wild-type DDR2 and its lung cancer mutants.
Iwai LK, Payne LS, Luczynski MT, Chang F, Xu H, Clinton RW, Paul A, Esposito EA, Gridley S, Leitinger B, Naegle KM, Huang PH
The Biochemical journal 2013 Sep 15;454(3):501-13
The Biochemical journal 2013 Sep 15;454(3):501-13
Discoidin domain receptor 2 (DDR2) regulates proliferation of endochondral cells in mice.
Kawai I, Hisaki T, Sugiura K, Naito K, Kano K
Biochemical and biophysical research communications 2012 Oct 26;427(3):611-7
Biochemical and biophysical research communications 2012 Oct 26;427(3):611-7
DDR2 plays a role in fibroblast migration independent of adhesion ligand and collagen activated DDR2 tyrosine kinase.
Herrera-Herrera ML, Quezada-Calvillo R
Biochemical and biophysical research communications 2012 Dec 7;429(1-2):39-44
Biochemical and biophysical research communications 2012 Dec 7;429(1-2):39-44
Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1.
Xu H, Raynal N, Stathopoulos S, Myllyharju J, Farndale RW, Leitinger B
Matrix biology : journal of the International Society for Matrix Biology 2011 Jan;30(1):16-26
Matrix biology : journal of the International Society for Matrix Biology 2011 Jan;30(1):16-26
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human DDR2 by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with human DDR2 untreated (-) or treated (+) with Calyculin A for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for DDR2 at approximately 139 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human DDR2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2538) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.