LS-C775410

antibody from LSBio

Targeting: SLC2A2 GLUT2

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C775410

Provider

LSBio

Product name

SLC2A2 / GLUT2 Antibody (Biotin) LS-C775410

Antibody type

Polyclonal

Description

Peptide Affinity Purified

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Biotin

Storage

Store at -20°C.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Rat Liver showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~37kDa.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~72kDa.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Rat Liver showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~37kDa.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~57.5kDa GLUT2 protein using Rabbit Anti-GLUT2 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~57.5kDa. Other Band(s): ~72kDa.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GLUT2 Polyclonal Antibody. Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GLUT2 Antibody (D) Composite.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GLUT2 Polyclonal Antibody. Tissue: Colon cancer cell line (HT-29). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GLUT2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm, membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GLUT2 Antibody (D) Composite.