MAB1414

antibody from R&D Systems

Targeting: SLC2A2 GLUT2

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1414

Provider

R&D Systems

Product name

Human Glut2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Recognizes human Glut2 expression on human Glut2-transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P11168


Isotype

IgG

Antibody clone number

199017

Vial size

200 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Mutations in SLC2A2 gene reveal hGLUT2 function in pancreatic β cell development.

Michau A, Guillemain G, Grosfeld A, Vuillaumier-Barrot S, Grand T, Keck M, L'Hoste S, Chateau D, Serradas P, Teulon J, De Lonlay P, Scharfmann R, Brot-Laroche E, Leturque A, Le Gall M
The Journal of biological chemistry 2013 Oct 25;288(43):31080-92

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Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.

Kornete M, Beauchemin H, Polychronakos C, Piccirillo CA
Autoimmunity 2013 Jun;46(4):259-68

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Ongoing beta-cell turnover in adult nonhuman primates is not adaptively increased in streptozotocin-induced diabetes.

Saisho Y, Manesso E, Butler AE, Galasso R, Kavanagh K, Flynn M, Zhang L, Clark P, Gurlo T, Toffolo GM, Cobelli C, Wagner JD, Butler PC
Diabetes 2011 Mar;60(3):848-56

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Isolation and characterization of a stem cell population from adult human liver.

Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G
Stem cells (Dayton, Ohio) 2006 Dec;24(12):2840-50

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Glut2 in Human Pancreas. Glut2 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Mouse Anti-Human Glut2 Monoclonal Antibody (Catalog # MAB1414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Glut2 in Human Pancreatic Cancer Tissue. Glut2 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human Glut2 Monoclonal Antibody (Catalog # MAB1414) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 Monoclonal Antibody (Catalog # MAB1414, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.