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LearnH00001736-M04
antibody from Abnova Corporation
Targeting: DKC1
Cbf5, DKC, dyskerin, NAP57, NOLA4, XAP101
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- H00001736-M04 - Provider product page
- Provider
- Abnova Corporation
- Product name
- DKC1 monoclonal antibody (M04), clone 3E10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant DKC1.
- Antigen sequence
MADAEVIILPKKHKKKKERKSLPEEDVAEIQHAEE
FLIKPESKVAKLDTSQWPLLLKNFDKLNVRTTHYT
PLACGSNPLKREIGDYIRTGFINLDKPSNPSSHEV
VAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERAT
RLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALET
LTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPE
RRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQE
LRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDES
YLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKI
MLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALM
TTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPK
ASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYS
ESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESES
DETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPG
DGDSDTTKKKKKKKKAKEVELVSE- Isotype
- IgG
- Antibody clone number
- 3E10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DKC1 expression in transfected 293T cell line by DKC1 monoclonal antibody (M04), clone 3E10.Lane 1: DKC1 transfected lysate (Predicted MW: 57.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DKC1 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
