Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF238 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGF-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human FGF-6 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human (rh) FGF-4 is observed, less than 5% cross-reactivity with rhFGF-9 is observed, and less than 2% cross-reactivity with FGF-acidic, rhFGF-5, and rhFGF-7 is observed. In direct ELISAs, less than 1% cross-reactivity with rh beta-ECGF, recombinant mouse (rm) FGF-8b, and rmFGF-8c is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P10767
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Embryonic myogenesis pathways in muscle regeneration.
FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
Zhao P, Hoffman EP
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Feb;229(2):380-92
Developmental dynamics : an official publication of the American Association of Anatomists 2004 Feb;229(2):380-92
FGF6 mediated expansion of a resident subset of cells with SP phenotype in the C2C12 myogenic line.
Israeli D, Benchaouir R, Ziaei S, Rameau P, Gruszczynski C, Peltekian E, Danos O, Garcia L
Journal of cellular physiology 2004 Dec;201(3):409-19
Journal of cellular physiology 2004 Dec;201(3):409-19
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by FGF-6 and Neutralization by Human FGF-6 Antibody. Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF238). The ND50 is typically 0.2-1 µg/mL in the presence of heparin (1 µg/mL).