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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB238 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FGF-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human FGF-6 in ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant human (rh) FGF-5 and less than 10% cross-reactivity with rhFGF-4 is observed. Does not neutralize the activity of FGF acidic, FGF basic, rhFGF-4, or rhFGF-5.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P10767- Isotype
- IgG
- Antibody clone number
- 27028
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Increased expression of fibroblast growth factor 6 in human prostatic intraepithelial neoplasia and prostate cancer.
Ropiquet F, Giri D, Kwabi-Addo B, Mansukhani A, Ittmann M
Cancer research 2000 Aug 1;60(15):4245-50
Cancer research 2000 Aug 1;60(15):4245-50
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by FGF-6 and Neutralization by Human FGF-6 Antibody. Recombinant Human FGF-6 (Catalog # 238-F6) stimulates proliferation in the the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF-6 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF-6 Monoclonal Antibody (Catalog # MAB238). The ND50 is typically 1-4 µg/mL in the presence of heparin (1 µg/mL).
