AF640

antibody from R&D Systems

Targeting: EPHA3 ETK, ETK1, HEK, HEK4, TYRO4

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF640

Provider

R&D Systems

Product name

Mouse EphA3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse EphA3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphA4, rmEphA8, recombinant rat EphA5, and rmEphA6 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q8BRB1


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

EphA3 null mutants do not demonstrate motor axon guidance defects.

Vaidya A, Pniak A, Lemke G, Brown A
Molecular and cellular biology 2003 Nov;23(22):8092-8

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EphA3 null mutants do not demonstrate motor axon guidance defects.

Vaidya A, Pniak A, Lemke G, Brown A
Molecular and cellular biology 2003 Nov;23(22):8092-8

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

EphA3 in Mouse Embryo. EphA3 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse EphA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.