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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3005 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3005, RRID:AB_1574702
- Product name
- EPHA3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of EPHA3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of cell surface targets through meta-analysis of microarray data.
Identification of a tumor-specific shared antigen derived from an Eph receptor and presented to CD4 T cells on HLA class II molecules.
Molecular cloning of HEK, the gene encoding a receptor tyrosine kinase expressed by human lymphoid tumor cell lines.
Isolation and characterization of a novel receptor-type protein tyrosine kinase (hek) from a human pre-B cell line.
Haeberle H, Dudley JT, Liu JT, Butte AJ, Contag CH
Neoplasia (New York, N.Y.) 2012 Jul;14(7):666-9
Neoplasia (New York, N.Y.) 2012 Jul;14(7):666-9
Identification of a tumor-specific shared antigen derived from an Eph receptor and presented to CD4 T cells on HLA class II molecules.
Chiari R, Hames G, Stroobant V, Texier C, Maillère B, Boon T, Coulie PG
Cancer research 2000 Sep 1;60(17):4855-63
Cancer research 2000 Sep 1;60(17):4855-63
Molecular cloning of HEK, the gene encoding a receptor tyrosine kinase expressed by human lymphoid tumor cell lines.
Wicks IP, Wilkinson D, Salvaris E, Boyd AW
Proceedings of the National Academy of Sciences of the United States of America 1992 Mar 1;89(5):1611-5
Proceedings of the National Academy of Sciences of the United States of America 1992 Mar 1;89(5):1611-5
Isolation and characterization of a novel receptor-type protein tyrosine kinase (hek) from a human pre-B cell line.
Boyd AW, Ward LD, Wicks IP, Simpson RJ, Salvaris E, Wilks A, Welch K, Loudovaris M, Rockman S, Busmanis I
The Journal of biological chemistry 1992 Feb 15;267(5):3262-7
The Journal of biological chemistry 1992 Feb 15;267(5):3262-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of EPHA3 polyclonal antibody (Cat # PAB3005) in NCI-H460 (Land 1), 293 (Land 2) cell line lysates (35 ug/lane). EPHA3 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with EPHA3 polyclonal antibody (Cat # PAB3005) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
