PAB3006

antibody from Abnova Corporation

Targeting: EPHA3 ETK, ETK1, HEK, HEK4, TYRO4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3006

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3006, RRID:AB_1574701

Product name

EPHA3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of EPHA3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of a tumor-specific shared antigen derived from an Eph receptor and presented to CD4 T cells on HLA class II molecules.

Chiari R, Hames G, Stroobant V, Texier C, Maillère B, Boon T, Coulie PG
Cancer research 2000 Sep 1;60(17):4855-63

---

Molecular cloning of HEK, the gene encoding a receptor tyrosine kinase expressed by human lymphoid tumor cell lines.

Wicks IP, Wilkinson D, Salvaris E, Boyd AW
Proceedings of the National Academy of Sciences of the United States of America 1992 Mar 1;89(5):1611-5

---

Isolation and characterization of a novel receptor-type protein tyrosine kinase (hek) from a human pre-B cell line.

Boyd AW, Ward LD, Wicks IP, Simpson RJ, Salvaris E, Wilks A, Welch K, Loudovaris M, Rockman S, Busmanis I
The Journal of biological chemistry 1992 Feb 15;267(5):3262-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of EPHA3 polyclonal antibody (Cat # PAB3006) in CHO cell lysate. EPHA3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of EPHA3 (arrow) using EPHA3 polyclonal antibody (Cat # PAB3006). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA3 gene (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human placenta tissue reacted with EPHA3 polyclonal antibody (Cat # PAB3006), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)