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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [3]
- ELISA [1]
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- Product number
- H00002042-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002042-M01, RRID:AB_538592
- Product name
- Mouse Monoclonal EphA3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EPHA3 - EPH receptor A3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references EPHA3 regulates the multidrug resistance of small cell lung cancer via the PI3K/BMX/STAT3 signaling pathway.
Peng J, Wang Q, Liu H, Ye M, Wu X, Guo L
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Sep;37(9):11959-11971
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Sep;37(9):11959-11971
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - Analysis of EPHA3 over-expressed 293 cell line, cotransfected with EPHA3 Validated Chimera RNAi ( Cat # H00002042-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EPHA3 monoclonal antibody (M01) clone 3A12 (Cat # H00002042-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - analysis of EPHA3 expression in transfected 293T cell line by EPHA3 monoclonal antibody (M01), clone 3A12. Lane 1: EPHA3 transfected lysate (110.1 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EphA3 Antibody (3A12) [H00002042-M01] - EPHA3 monoclonal antibody (M01), clone 3A12. Analysis of EPHA3 expression in human spleen.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: EphA3 Antibody (3A12) [H00002042-M01] - Detection limit for recombinant GST tagged EPHA3 is approximately 0.03ng/ml as a capture antibody.
