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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AER-013-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4)brain membranes: - 1,2. Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200).3,4. Anti-EphA3 (extracellular) Antibody , preincubated with EphA3 (extracellular) Blocking Peptide (#BLP-ER013).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human Malme-3M melanoma cell line lysate (lanes 3 and 7) and human HT-29 colorectal adenocarcinoma cell line lysate (lanes 4 and 8): - 1-4. Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200).5-8. Anti-EphA3 (extracellular) Antibody , preincubated with EphA3 (extracellular) Blocking Peptide (#BLP-ER013).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of EphA3 in rat and mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections with Anti-EphA3 (extracellular) Antibody (#AER-013), (1:200), followed by goat Anti-rabbit-AlexaFluor-488. A. EphA3 staining (green) in rat hippocampal CA1 region is detected in the pyramidal layer (P). B. In mouse hippocampal CA1 region, EphA3 immunoreactivity (green) is observed in the pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of EphA3 in live intact human Jurkat T-cell leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-EphA3 (extracellular) Antibody (#AER-013), (5 µg) + goat- Anti-rabbit-FITC.
